All 20 subjects completed the study and their demographic characteristics are reported in Table 1.
All the instrumental examinations were performed in the same medical office , at an average temperature of 23 ± 1.2 ° C and at an average humidity of 54 ± 1.7%, between 10 and 14 o’clock in the morning. The measurements were always made by the same experimenter. The last instrumental survey was performed on average, since the last serotin application, after 16 ± 2.2 hours.

   Demographic characteristics of the patients
 
N= 20
   Male 9 (45%)
   Female 11 (55%)
   Age (range) 20 – 36
   Age (mean and SD) 28,8 ± 4,47
   Mild acne 12 (60%)
  Moderate acne 8 (40%)

CLINICAL EVALUATION 

The product has been shown to progressively reduce the number of acne lesions during treatment (Table 2). The most important action was on pustules where the decrease reached statistical significance after 30 days of treatment. Also the TLC confirms the progressive decrease in lesions reaching statistical significance after 30 days (table 2).

PG6 ACTIVE AA- BET C® t0 t15gg t30g
Papule number measurement 19,55 ± 5,11 18,8 ± 4,38 18,4 ± 4,01
p-value   0,175 0,055
Pustule number measurement 9,45 ± 3,67 8,9 ± 3,37 6,95 ± 1,70
p-value   0,221 0,003
Comedo number measurement 7,6 ± 3,39 7,05 ± 1,79 6,65 ± 2,05
p-value   0,213 0,115
TLC (Total Lesions Count) 36,6 ± 8,37 34,75 ± 5,44 32 ± 4,76
p-value   0,072 0,004

INSTRUMENTAL EVALUATION

Sebometric value
The mean sebometry values were reduced by about 6%, reaching statistical significance after 30 days of treatment (Table 3).

PG6 ACTIVE AA- BET C® t0 t15gg t30gg
Sebometric measurement 73,85 ± 19,1 69,7 ± 18,4 67,95 ± 18,2
Baseline change from t0 (mean and SD)
  -4,1 ± 23,3 -5,9 ± 15,0
Percentage change from t0 (mean)
  -0,90 % -6,18 %
p-value   0,218 0,048

pHmetry values
The mean pHmetry values remained constant, demonstrating that the treatment maintained the cutaneous pH values in the physiological range (table 4).

PG6 ACTIVE AA- BET C® t0 t15gg t30gg
pH measurement 5,52 ± 0,25 5,47 ± 0,27 5,53 ± 0,20
Baseline change from t0 (mean and SD)
  -0,049 ± 0,20 0,0050 ± 0,26
Percentage change from t0 (mean)
  -0,8 % 0,23 %
p-value   0,146 0,467

Colorimetric values
Papular lesions reduced their erythema statistically significantly after 30 days after application of the product, demonstrating that it has anti-inflammatory activity (table 5).

PG6 ACTIVE AA- BET C® t0 t15gg t30gg
Papular colorimetric measurement 308,4 ± 41,3 295,4 ± 36,41 288,5 ± 28,0
Baseline change from t0 (mean and SD)
  -12, ± 58,53 -19, ± 38,3
Percentage change from t0 (mean)
  -2,4 % -5,4 %
p-value   0,167 0,016