Test Item

Designation in Test Facility: 14121110N
Name

SESAMULS® OW (Polyglyceryl-3 Cetyl Ether, Sesame Oil, Malic Acid)

Batch No. C0092

Storage

The test item was stored in a closed vessel dark and dry at room temperature.

Preparation
Due to the poor solubility of the test item, the water-accommodated fraction (WAF) of the concentrations to be tested was prepared. This was done by weighing the nominal loads (1 mg/L, 10 mg/L and 100 mg/L), adding the appropriate amount of nutrient medium (demineralised water enriched with minerals but without algae) and shaking for 24 hours. The resulting solutions were filtrated through 0.45 μm filters.

Positive Control

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a separate reference test (GLP study).

Test System

Specification

Unicellular freshwater green alga.

Genus Desmodesmus
Species subspicatus
SAG Strain Number 86.81
Taxonomic position

Chlorophyta – Chlorophyceae 

Origin and Culture

The culture of Desmodesmus subspicatus was obtained in February 2019 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 7 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.

Chemicals

All solutions were sterilised before use.

Composition of the solutions:

Stock solution I

NH4Cl

1500 mg

MgCl2*6H2O 

1200 mg

CaCl2*2H2O 

1800 mg

MgSO4*7H2O 

1500 mg

KH2PO4 

180 mg

H2O deionized 

ad 1000 mL
 
Stock solution II

FeCl3*6H2O 

64 mg

Na2EDTA*2H2O

100 mg

H2O deionized 

ad 1000 mL
 
Stock solution III

H3BO3

185 mg

MnCl2*4H2O

415 mg

ZnCl2 

3 mg

CoCl2*6H2O 

1.5 mg

CuCl2*2H2O 

0.01 mg

Na2MoO4*2H2O 

7 mg

H2O deionized 

ad 1000 mL
 
Stock solution IV

NaHCO3

50 mg

H2O deionized 

ad 1000 mL
 
Pre-culture-medium
Stock solution I 10.0 mL
Stock solution II 1.0 mL
Stock solution III 1.0 mL
Stock solution IV 1.0 mL

H2O deionized 

 ad 1000 mL
 
Nutrient-medium (10-fold concentrated)
Stock solution I 100 mL
Stock solution II 10 mL
Stock solution III 10 mL
Stock solution IV 10 mL

H2O deionized 

 ad 1000 mL