Tables

IC-Values
In the following tables, the IC values (given in mg/L) which were measured in the samples of the front scrubber flasks are stated.

Table 3.1-a IC Values in mg/L Apparatus Blanks, Controls, front scrubber

   Day Apparatus blank 1 Apparatus blank 2 Control 1 Control 2
   0 0.74 1.20 1.28 1.80
   2 2.70 4.08 10.24 9.09
   4 4.14 5.18 21.03 17.16
   7 4.69 6.07 35.74 29.69
   9 5.79 7.64 42.17 37.43
   11 6.33 8.34 43.62 41.19
   14 7.24 11.36 51.94 50.40
   18 10.24 13.27 59.40 57.75
   23 12.48 17.61 65.40 66.07
   29 16.60 22.49 75.91 76.79

Table 3.1-b IC Values in mg/L Positive Control, Test Flasks, front scrubber

   Day Positive Control 1 Positive Control 2 Test 1* Test 2 Abiotic Control Toxicity Control
   0 1.42 1.00 0.60 4.84 0.75 1.29
   2 7.51 11.91 31.38 14.16 4.34 16.87
   4 55.54 109.72 78.40 36.70 6.47 156.58
   7 193.95 251.25 140.97 139.48 6.69 303.64
   9 264.64 278.75 161.33 179.75 8.87 350.32
   11 270.29 282.51 170.80 198.64 9.42 364.91
   14 289.95 288.15 196.63 238.69 9.60 401.77
   18 318.81 322.09 210.51 270.90 12.31 409.49
   23 335.71 338.78 228.67 295.58 13.76 444.85
   29 347.17 353.45 253.77 339.44 17.73 550.91

*At the end of the test, an untight seal in the cap of test flask 2 was located.
Therefore the measured IC values were significant lower than in test
replicate 2. Test replicate 1 was not used for evaluation.

In the following tables, the IC values which were measured in the samples of the back scrubber flasks are stated.

Table 3.1-c IC Values in mg/L Controls, Apparatus Blanks, back scrubber

   Day Apparatus blank 1 Apparatus blank 2 Control 1 Control 2
   0 0.86 1.88 0.72 1.29
   29 10.06 12.28 3.22 4.31

Table 3.1-d IC Values in mg/L Positive Control, Test Flasks, back scrubber

Day Positive Control 1 Positive Control 2 Test 1 Test 2 Abiotic Control Toxicity Control
0 1.19 0.73 0.75 1.71 0.39 1.72
29 5.29 3.20 5.45 9.29 3.55 3.04

Net IC
For each flask, the IC value which was measured at the start of the test (d = 0) was subtracted from all following measurements. The net IC was calculated using this corrected measurement value and subtracting the mean IC value of the apparatus blanks of that sampling date.

The net IC values are presented in the following table.
Table 3.1-e Net IC-values in mg/L front scrubber flasks

   Day Control 1 Control 2 Positive Control 1 Positive Control 2 Test 1 Test 2 Abiotic Control Toxicity Control
   0 1.3 1.8 1.4 1.0 0.6 4.8 0.8 1.3
   2 7.8 6.7 5.1 9.5 29.0 11.7 1.9 14.5
   4 17.3 13.5 51.9 106.0 74.7 33.0 2.8 152.9
   7 31.3 25.3 189.5 246.8 136.6 135.1 2.3 299.2
   9 36.4 31.7 258.9 273.0 155.6 174.0 3.1 344.6
   11 37.3 34.8 263.9 276.1 164.4 192.3 3.1 358.5
   14 43.6 42.1 281.6 279.8 188.3 230.4 1.3 393.4
   18 48.6 47.0 308.0 311.3 199.7 260.1 1.5 398.7
   23 51.3 52.0 321.6 324.7 214.6 281.5 -0.3 430.8
   29 57.3 58.2 328.6 334.9 235.2 320.9 -0.8 532.3

Table 3.1-f Net IC-values in mg/L back scrubber flasks

Day Control 1 Control 2 Positive Control 1 Positive Control 2 Test 1 Test 2 Abiotic Control Toxicity Control
0 0.7 1.3 1.2 0.7 0.7 1.7 0.4 1.7
29 -6.6 -5.5 -4.5 -6.6 -4.4 -0.5 -6.3 -6.8

Negative values occur, when the apparatus blank was higher than the respective treatment. As the measured values in these blanks as well as in the abiotic control are very low, measurement uncertainties lead to negative degradation values in the abiotic control.

pH
In the following table, the pH at the end of the test (before addition of HCl) is given:

Day Control 1 Control 2 Positive Control 1 Positive Control 2 Test 1 Test 2 Abiotic Control Toxicity Control
28 7.4 7.4 7.2 7.2 7.4 7.4 6.8 7.2

Equations

Emitted Carbon in mg/L
Emitted Carbon in mg/L test solution at time t is calculated using the following equation:

emittC
emitted carbon in mg/L test solution
IC(t)
inorganic carbon in mg/L NaOH at time t
IC(0)
inorganic carbon in mg/L NaOH at the start of the test
VolNaOH (t)
remaining volume NaOH in L in the scrubber at time t (Volume at t = 0 (here: 0.1 L) – Ʃ (all sample volumes up to time t))
VolTestVessel
test vessel volume in L (here: 1.5)

For day 29, the IC content of both scrubber flasks was taken into account.
Calculation of emitted carbon is necessary for the assessment of validity. The value obtained with this equation is multiplied with 3.667 (44/12) in order to obtain emitted CO2.

Degradation in %
The percentage biodegradation in the test flasks was calculated from:

Degradation in positive control and toxicity flasks was calculated analogously.
Abiotic degradation was calculated from: