Findings – biodegradability – SESAMULS® OW
FINDINGS
In the following tables, the IC values (given in mg/L) which were measured in the samples of the front scrubber flasks are stated.
Day |
Apparatus blank 1 |
Apparatus blank 2 |
Control 1 |
Control 2 |
0 |
0.74 |
1.20 |
1.28 |
1.80 |
2 |
2.70 |
4.08 |
10.24 |
9.09 |
4 |
4.14 |
5.18 |
21.03 |
17.16 |
7 |
4.69 |
6.07 |
35.74 |
29.69 |
9 |
5.79 |
7.64 |
42.17 |
37.43 |
11 |
6.33 |
8.34 |
43.62 |
41.19 |
14 |
7.24 |
11.36 |
51.94 |
50.40 |
18 |
10.24 |
13.27 |
59.40 |
57.75 |
23 |
12.48 |
17.61 |
65.40 |
66.07 |
29 |
16.60 |
22.49 |
75.91 |
76.79 |
Table 3.1-a IC Values in mg/L Apparatus Blanks, Controls, front scrubber
Day |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
0 |
1.42 |
1.00 |
1.05 |
1.36 |
1.26 |
0.83 |
2 |
7.51 |
11.91 |
9.46 |
15.80 |
3.61 |
8.33 |
4 |
55.54 |
109.72 |
27.06 |
33.41 |
5.33 |
108.10 |
7 |
193.95 |
251.25 |
63.47 |
73.15 |
5.08 |
305.03 |
9 |
264.64 |
278.75 |
82.66 |
112.59 |
6.02 |
383.22 |
11 |
270.29 |
282.51 |
99.58 |
130.46 |
5.20 |
404.29 |
14 |
289.95 |
288.15 |
129.74 |
167.64 |
6.74 |
451.31 |
18 |
318.81 |
322.09 |
165.64 |
205.29 |
8.03 |
486.72 |
23 |
335.71 |
338.78 |
211.05 |
236.45 |
9.69 |
545.05 |
29 |
347.17 |
353.45 |
270.74 |
290.66 |
13.81 |
586.64 |
Table 3.1-b IC Values in mg/L Positive Control, Test Flasks, front scrubber
In the following tables, the IC values which were measured in the samples of the back scrubber flasks are stated.
Day |
Apparatus blank 1 |
Apparatus blank 2 |
Control 1 |
Control 2 |
0 |
0.86 |
1.88 |
0.72 |
1.29 |
29 |
10.06 |
12.28 |
3.22 |
4.31 |
IC Values in mg/L Controls, Apparatus Blanks, back scrubber
Day |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
0 |
1.19 |
0.73 |
1.42 |
1.45 |
1.76 |
1.26 |
29 |
5.29 |
3.2 |
3.61 |
5.94 |
9.35 |
3.27 |
IC Values in mg/L Positive Control, Test Flasks, back scrubber
Net IC
For each flask, the IC value which was measured at the start of the test (d = 0) was subtracted from all following measurements. The net IC was calculated using this corrected measurement value and subtracting the mean IC value of the apparatus blanks sampling date.
The net IC values are presented in the following table.
Day |
Control 1 |
Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Contro l |
0 |
1.3 |
1.8 |
1.4 |
1.0 |
1.1 |
1.4 |
1.3 |
0.8 |
2 |
7.8 |
6.7 |
5.1 |
9.5 |
7.0 |
13.4 |
1.2 |
5.9 |
4 |
17.3 |
13.5 |
51.9 |
106.0 |
23.4 |
29.7 |
1.6 |
104.4 |
7 |
31.3 |
25.3 |
189.5 |
246.8 |
59.1 |
68.7 |
0.7 |
300.6 |
9 |
36.4 |
31.7 |
258.9 |
273.0 |
76.9 |
106.8 |
0.3 |
377.5 |
11 |
37.3 |
34.8 |
263.9 |
276.1 |
93.2 |
124.1 |
-1.2 |
397.9 |
14 |
43.6 |
42.1 |
281.6 |
279.8 |
121.4 |
159.3 |
-1.6 |
443.0 |
18 |
48.6 |
47.0 |
308.0 |
311.3 |
154.9 |
194.5 |
-2.8 |
475.9 |
23 |
51.3 |
52.0 |
321.6 |
324.7 |
197.0 |
222.4 |
-4.4 |
531.0 |
29 |
57.3 |
58.2 |
328.6 |
334.9 |
252.2 |
272.1 |
-4.8 |
568.1 |
Net IC-values in mg/L front scrubber flasks
Day |
Control 1 |
Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
0 |
0.7 |
1.3 |
1.2 |
0.7 |
1.4 |
1.5 |
1.8 |
1.3 |
29 |
-6.6 |
-5.5 |
-4.5 |
-6.6 |
-6.2 |
3.9 |
-0.5 |
-6.5 |
Net IC-values in mg/L back scrubber flasks
Negative values occur, when the apparatus blank was higher than the respective treatment. As the measured values in these blanks as well as in the abiotic control are very low, measurement uncertainties lead to negative degradation values in the abiotic control.
In the following table, the pH at the end of the test (before addition of HCl) is given:
Day |
Control 1 |
Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
28 |
7.4 |
7.4 |
7.2 |
7.2 |
7.4 |
7.4 |
6.8 |
7.2 |
pH Test flasks on day 28
Equations
Emitted Carbon in mg/L
Emitted Carbon in mg/L test solution at time t is calculated using the following equation:
emittC | emitted carbon in mg/L test solution |
IC (t) | inorganic carbon in mg/L NaOH at time t |
IC(0) | inorganic carbon in mg/L NaOH at the start of the test |
VolNaOH (t) | remaining volume NaOH in Lin thescrubber at time t (Volume at t=0(here:0.1L) – ∑ (all sample volumes up to time t)) |
VolTestVessel | test vessel volume in L (here:1.5) |
Calculation of emitted carbon is necessary for the assessment of validity. The value obtained with this equation is multiplied with 3.667 (44/12) in order to obtain emitted CO2.
Degradation in %
The percentage biodegradation in the test flasks was calculated from:
Degradation in positive control and toxicity flasks was calculated analogously. Abiotic degradation was calculated from:
Calculation Results
Emitted Carbon in mg/L
In the following table, the calculated emitted carbon is presented.
Day |
Control 1 |
Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
2 |
0.43 |
0.32 |
0.24 |
0.56 |
0.40 |
0.79 |
0.00 |
0.34 |
4 |
1.05 |
0.76 |
3.29 |
6.86 |
1.46 |
1.85 |
0.02 |
6.77 |
7 |
1.94 |
1.52 |
12.17 |
15.90 |
3.75 |
4.36 |
-0.04 |
19.39 |
9 |
2.25 |
1.91 |
16.48 |
17.41 |
4.86 |
6.75 |
-0.06 |
24.11 |
11 |
2.28 |
2.09 |
16.63 |
17.43 |
5.84 |
7.77 |
-0.15 |
25.15 |
14 |
2.65 |
2.52 |
17.56 |
17.47 |
7.54 |
9.90 |
-0.18 |
27.71 |
18 |
2.93 |
2.80 |
19.01 |
19.24 |
9.54 |
11.98 |
-0.25 |
29.46 |
23 |
3.07 |
3.08 |
19.64 |
19.85 |
12.02 |
13.56 |
-0.35 |
32.52 |
29 |
2.92 |
2.98 |
19.47 |
19.77 |
14.73 |
16.07 |
-0.51 |
33.90 |
Emitted carbon in mg/L
Degradation Values
In the following table the percentage biodegradation is presented:
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Test 1 |
Test 2 |
Mean |
Abiotic Control |
Toxicity Control |
2 |
0.7 |
0.9 |
0.1 |
0.1 |
2.1 |
1.1 |
0.0 |
0.1 |
4 |
12.0 |
29.9 |
20.9 |
2.8 |
4.7 |
3.7 |
0.1 |
14.8 |
7 |
52.3 |
71.0 |
61.7 |
10.2 |
13.0 |
11.6 |
0.2 |
44.6 |
9 |
72.2 |
76.8 |
74.5 |
14.0 |
23.2 |
18.6 |
0.3 |
55.6 |
11 |
72.4 |
76.4 |
74.4 |
18.4 |
27.7 |
23.1 |
0.8 |
58.0 |
14 |
75.0 |
74.6 |
74.8 |
24.9 |
36.3 |
30.6 |
0.9 |
63.5 |
18 |
80.9 |
82.1 |
81.5 |
33.6 |
45.2 |
39.4 |
1.2 |
67.2 |
23 |
83.0 |
84.1 |
83.6 |
45.0 |
52.0 |
48.5 |
1.7 |
74.4 |
29 |
82.8 |
84.3 |
63.6 |
59.3 |
65.2 |
62.3 |
2.5 |
78.2 |
Degradation values in %
Degradation Graph