VALIDITY

Growth

Increase Factor

The cell concentration in the control should increase by a factor of at least 16 within 72h.

 

Daily Growth Rates
Mean coefficient of variation of daily growth rates should be 35% at the most.

Coefficient of variation of average growth rate during the whole test period should be 7% at the most.

 

Values and assessment

The daily growth rates of the controls were calculated. Means, standard deviations and coefficients of variation were determined. Values and assessment can be found in the following tables.

 

Table 8.1-a Daily Growth Rates of the Controls

Growth rates days 0 – 1 days 1 – 2 days 2 – 3 CV of sectional daily growth rates days 0 – 3
   Replicate 1 1.705 1.291 1.361 15% 1.45
   Replicate 2 1.705 1.431 1.295 14% 1.48
   Replicate 3 1.705 1.591 1.299 14% 1.53
   Replicate 4 1.792 1.642 1.337 15% 1.59
   Replicate 5 1.794 1.488 1.307 21% 1.58
   Replicate 6 1.792 1.626 1.255 18% 1.56
   Mean 1.774 1.512 1.309 16% 1.531
   Standard deviation 0.094 0.136 0.036   0.056
   CV 5% 9% 3%   4%

CV = Coefficient of Variation

Growth Curve

During the test exponential growth was given.

Change of pH

The pH of the control should not fluctuate by more than 1.5 units. The change was 0.8 units.