Product tested

SESAMULS WO (Polyglyceryl-6 Pentaoleate, Sesame Oil, Malic Acid)

The carbon content of 73.8 % was determined by elemental analysis. The test item was used directly, in amounts according to its carbon content.

Positive Control
Aniline (Phenylamine, C6H5NH2, CAS-No. 62-53-3) was used as readily bio-degradable positive control. A stock solution containing 2127.8 mg/L in deionised water was prepared and its organic carbon content was measured with 1729.9 ppm, corresponding to an organic carbon content of the positive control of 81.3 %.

Test System

Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly domestic sewage.

Source and Pre-Treatment

The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 09. Jan 2019, batch no: 20150119.

The sludge was filtrated, washed with tap water twice, then washed with and re-suspended in test medium. It was then aerated for > 12 hours. The dry matter was determined with 4880 mg suspended solids/litre.


All chemicals used in the test were “analytical grade” or otherwise proved suitable.

Stock solutions

Solution A
   Potassium dihydrogenephosphate (KH2PO4) 8.5 g
   Di-potassium hydrogenephosphate (K2HPO4) 21.75 g
   Di-sodiumhydrogenephosphate dihydrate (Na2HPO4*2H2O) 33.4g
   Ammonia chloride (NH4Cl) 0.5 g
   H2O demin. ad 1000 mL
   The pH was adjusted to 7.4 ± 0.1.  
Solution B
   Calcium chloride dihydrate (CaCl2*2H2O) 36.4 g
   H2O demin. ad 1000 mL
Solution C
   Magnesium sulfate heptahydrate (MgSO4*7H2O) 22.5 g
   H2O demin. ad 1000 mL
Solution D
   Iron(III) chloride hexahydrate (FeCl3*6H2O) 0.25 g
   Di-sodium-ethylendiamintetraacetate dihydrate (Na2EDTA*2H2O) 0.4 g
   H2O demin. ad 1000 mL

Test Medium

The medium was freshly prepared.

   Solution A 10 mL
   Solution B 1 mL
   Solution C 1 mL
   Solution D 1 mL
   H2O demin. ad 1000 mL

Sodium Hydroxide
NaOH, 0.25-m solution, used for trapping of emitted carbon dioxide.
NaOH, 1.5-m solution, used for scrubbing of purified air.

Mercury Chloride
HgCl2, used for poisoning of abiotic flasks

Barium hydroxide
Ba(OH)2 solution, used for checking the purified air (saturated solution ,1:3 diluted).

Hydrochloric Acid
HCl, 2-m solution, used for driving off dissolved CO2 on day 28.

Reference Items for Carbon Determination
Potassium hydrogenphthalate for TC, Na2CO3 and NaHCO3 for IC.

Test Vessels
All glassware was cleaned with the laboratory cleaning agent and then rinsed with tap water (thrice), diluted HCL (once), tap water (thrice) and deionised water (thrice).
2000 mL-SCHOTT-flasks were used as test vessels, 100 mL scrubber flasks as absorbent vessels.